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mcherry paxillin  (Addgene inc)


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    Structured Review

    Addgene inc mcherry paxillin
    Mcherry Paxillin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcherry+paxillin/pmCherry+Paxillin+(Plasmid+%2350526)/bio_rxiv__2025__11__17__688923-176-0-19
    Average 93 stars, based on 32 article reviews
    mcherry paxillin - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    other:

    Article Title: Spatial organization of PI3K-PI(3,4,5)P3-AKT signaling by focal adhesions
    Article Snippet: LYN11-FRB-ECFP (#38003), mCherry-FKBP-INPP4B (#116864), NES-EGFP-cPHx3 (#116855), mCherry-FKBP-PTEN (#116866), mCherry-CRY2-iSH2 (#66839), mCherry-α-tubulin (#21043), mCherry-paxillin (#50526), mCherry-FAK (#55122), mCherry-vinculin (#55159) were obtained from Addgene.

    Article Title: Spatial proteomics of ER tubules reveals CLMN, an ER-actin tether at focal adhesions that promotes cell migration
    Article Snippet: mCherry-paxillin , Addgene 50526 , RRID:Addgene_50526.

    Article Title: Consolidation of cell-ECM adhesion through direct talin-mediated actin linkage is essential for mouse embryonic morphogenesis
    Article Snippet: Cells were then co-transfected with F-tractin-GFP (Addgene, 58473) and mCherry-paxillin (Addgene, 80029) using Lipofectamine 3000 (Invitrogen, L3000008) according to the manufacturer’s instructions.

    Article Title: Non-muscle myosin II isoforms orchestrate substrate stiffness sensing to promote cancer cell contractility and migration.
    Article Snippet: The stiffening of the extracellular matrix (ECM) during tumor progression results in an increase in cancer cell motility.. In cell migration, two major isoforms of non-muscle myosin II (NMII), NMIIA and NMIIB, are expressed and assembled into the cytoskeleton.. However, the isoform-specific regulatory roles of NMIIA and NMIIB as well as the underlying mechanisms in response to mechanical cues of the ECM are still elusive.

    Article Title: Spatial organization of PI3K-PI(3,4,5)P 3 -AKT signaling by focal adhesions.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER u-track Jaqaman et al.84 https://github.com/DanuserLab/u-track BoundaryTrack Taihei Fujimori https://github.com/fjmrt/BoundaryTrack TrackMate 7 Ershov et al.85 https://imagej.net/plugins/trackmate/ InferenceMAP El Beheiry et al.86 https://research.pasteur.fr/en/software/ inferencemap bubblebath Adam Danz https://www.mathworks.com/ matlabcentral/fileexchange/70348 MSDanalyzer Tarantino et al.87 https://tinevez.github.io/msdanalyzer e4 Molecular Cell 84, 1–18.e1–e9, November 21, 2024 LYN11-FRB-ECFP (#38003), mCherry-FKBP-INPP4B (#116864), NES-EGFP-cPHx3 (#116855), mCherry-FKBP-PTEN (#116866), mCherry-CRY2-iSH2 (#66839), mCherry-paxillin (#50526), mCherry-FAK (#55122), mCherry-vinculin (#55159) were obtained from Addgene.

    Imaging:

    Article Title: Consolidation of cell-ECM adhesion through direct talin-mediated actin linkage is essential for mouse embryonic morphogenesis.
    Article Snippet: Fluorescence Recovery After Photobleaching (FRAP) live imaging PrimaryMEFs were cultured in 0.1% gelatin-coated tissue culture dishes to be 70–90% confluent before plasmid transfection. .. Cells were then cotransfected with F-tractin-GFP (Addgene, 58473) and mCherry-paxillin (Addgene, 80029) using Lipofectamine 3000 (Invitrogen, L3000008) according to themanufacturer’s instructions.At 24 hpost-transfection, cells were trypsinized andplated onto0.1 mg/mlfibronectin-coatedglass bottom dishes (MatTek, P35G-0-14-C) using imagingmedia (same as the one used in TIRFM live imaging) for either 90min or 180min at 37 °C before imaging live. .. Cells were imaged using an Olympus Fluoview FV1000 Laser Scanning Inverted Confocal Microscope with a 60X water immersion objective.

    Plasmid Preparation:

    Article Title: ERK builds a population of short-lived nascent adhesions that produce persistent edge protrusion and cell migration
    Article Snippet: .. mCherry-paxillin (#50526), mCherry-talin (#80026), F-tractin-EGFP (#58473), FAT-FAK biosensor (#78303), and mT-Sapphire-C1 (#54545), and pSpCas9(BB)-2A-Puro vector (#62988) were obtained from Addgene. .. Lentivirus vector pLVX-shRNA1 was from Clontech.

    Article Title: Cell clusters adopt a collective amoeboid mode of migration in confined nonadhesive environments
    Article Snippet: The DH-PH domain of ARHGEF11 was amplified and cloned into CRY2PHR-mCherry. pCIBN(deltaNLS)-pmGFP (Addgene plasmid no. 26867; http://n2t.net/addgene:26867 ; RRID: Addgene_26867) ( ) and pCRY2PHR-mCherryN1 (Addgene plasmid no. 26866; http://n2t.net/addgene:26866 RRID: Addgene_26866) ( ) are a gift from C. Tucker. .. The H2B-RFP plasmid is a gift from the Hall lab. pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast is a gift from Meyer and colleagues ( ) (Addgene plasmid no. 85146; http://n2t.net/addgene:85146 ; RRID: Addgene_85146); mCherry-Paxillin is a gift from the Vignjevic lab; and pLentiblast-Paxillin-mTurquoise is a gift from Debnath and colleagues ( ) (Addgene plasmid no. 74206; http://n2t.net/addgene:74206 ; RRID: Addgene_74206). ..



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